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Year : 2017  |  Volume : 20  |  Issue : 3  |  Page : 307-312

Evaluation of various filling techniques in distal canals of mandibular molars instrumented with different single-file nickel-titanium systems

Department of Endodontics, Faculty of Dentistry, Cukurova University, Adana, Turkey

Date of Acceptance11-Sep-2015
Date of Web Publication3-Mar-2017

Correspondence Address:
A Dumani
Department of Endodontics, Faculty of Dentistry, Cukurova University, Adana
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1119-3077.178920

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Background and Aims: The aim of this study was to evaluate the quality of various filling techniques in distal canals of mandibular molars instrumented with different single-file nickel-titanium (NiTi) systems. Materials and Methods: A total of 150 distal roots of mandibular molar teeth were randomly assigned into three main groups and instrumented by using Reciproc (VDW, Munich, Germany), WaveOne (Dentsply Tulsa, Tulsa, OK, USA), or One Shape (MicroMega, Besancon, France) NiTi file systems. The roots were then treated using one of five filling techniques: (1) Matched-single-cone, (2) cold lateral compaction with matched gutta-percha (GP) cone, (3) Thermafil filling, (4) System B/Obtura II, and (5) lateral compaction with standardized GP cones. The roots were then sectioned at three levels (coronal, middle, and apical). Photographs were acquired under a stereomicroscope, and the percentage of GP-filled areas (PGFAs), percentage of sealer-filled areas (PSFAs), and voids were measured using the ImageJ software. Comparisons between groups were applied using Student’s t-test or one-way ANOVA for normally distributed data. The Mann-Whitney U-test or Kruskal-Wallis test was used when variables were not normally distributed. Results: Canals filled with the System B/Obtura showed the highest PGFA and lowest PSFA, whereas those filled with matched-single-cone showed the highest PSFA and lowest PGFA (P < 0.05). The cold lateral compaction with matched GP cone group, lateral compaction group, and Thermafil filling group showed no statistically significant differences in PSFA and PGFA (P > 0.05). Conclusions: System B/Obtura technique appears to be the best technique to properly fill root canals, whereas the matched-single-cone technique in oval-shaped distal canals of mandibular molars was inadequate.

Keywords: Reciprocating files, root canal fillings, rotary files

How to cite this article:
Dumani A, Yilmaz S, Yoldas O, Kuden C. Evaluation of various filling techniques in distal canals of mandibular molars instrumented with different single-file nickel-titanium systems. Niger J Clin Pract 2017;20:307-12

How to cite this URL:
Dumani A, Yilmaz S, Yoldas O, Kuden C. Evaluation of various filling techniques in distal canals of mandibular molars instrumented with different single-file nickel-titanium systems. Niger J Clin Pract [serial online] 2017 [cited 2017 Mar 29];20:307-12. Available from:

   Introduction Top

Instrumentation and filling techniques are both important parts of a successful root canal treatment.[1],[2] For instrumentation, the newly manufactured nickel-titanium (NiTi) file systems are claimed to be able to prepare and clean root canals using only one file. Two of these single-file systems; Reciproc (VDW, Munich, Germany) and WaveOne (Dentsply Tulsa, Tulsa, OK, USA) are used in a reciprocating motion and another single-file system One Shape (Micro Mega, Besancon, France) is used in a traditional, continuous, and rotational motion.

There are several proposed techniques to achieve adequate three-dimensional filling of the well-instrumented root canals.[3] One of the best-known technique is lateral compaction of cold gutta-percha (GP) which is often used as a standard to compare new techniques.[4] However, this method is reported to have possible disadvantages such as inhomogeneity, an increased risk of canal fracture, and poor adaptation to the canal walls.[4] The other widely used technique is the warm vertical compaction of GP which is made by System B endodontic heat source unit (SybronEndo, Redmond, WA, USA) combined with a thermoplasticized GP injection system, Obtura II (Obtura Spartan, Fenton, USA). These systems were effective for downpack and backfill filling of the root canal system but require relatively long time, and application can be difficult especially in molar teeth.[5]

Recently, corresponding GP cones that match the taper and diameter of the files have been manufactured. Filling with this system may be less complex and time-consuming because these instruments and cones attempt to prepare a root canal to a certain shape and then fill the canal using a single GP cone.[6] There are a few suggestions regarding the appropriate use of matching GP cones in a “single-cone technique” or in combination with accessory cones.[7] Because the volume of sealer required for the single-cone technique is much more than the volume necessary to complete a compaction technique.[8] Carrier-based obturators were manufactured for reduce the amount of sealer used during the filling. This filling technique provides to compact thermo plasticized GP and sealer both laterally and vertically more rapidly than other techniques.[4]

All filling methods aim the maximum volume of GP and a thin layer of sealer because the sealer may shrink during setting and dissolve over time, causing leakage.[9] It is well-known that the coronal and apical leakages have important effects on the long-term success of endodontic treatment.[10] The percentage of GP-filled areas (PGFAs) has been used as a measure of the quality of the root filling.[7] Therefore, the aim of this study was to compare different filling techniques in the distal canals of lower molars, instrumented by different single-file systems, and the canal area in terms of the percentage of GP, sealer, and voids. The hypothesis of this study was that there would be a difference between different obturation techniques for the percentage of GP and sealer.

   Materials and Methods Top

A total of 150 extracted human mandibular molar teeth were selected for the study which had been stored in 10% formalin. The extracted teeth were obtained from patients affected by dental caries or severe periodontal disease. The study was conducted in full accordance with the World Medical Association Declaration of Helsinki. The research protocol and the informed consent form were approved by the Bioethics Committee of the University of Cukurova.

The crowns and distal roots were separated using a high-speed water-cooled diamond bur and 150 distal roots were assigned randomly into three groups of 50 teeth each and enlarged with Reciproc or Wave One or One shape file system. A size 10 H-file (Dentsply Maillefer, Ballaigues, Switzerland) was introduced into each of the canal until it appeared at the apical foramen, and the working length was established by subtracting 1 mm from this measurement.

Instrumentation was performed by a single operator (A.D.) in accordance with the manufacturers’ recommendations. Moreover, all files were operated with a torque controlled motor (VDW Silver). Primary Wave One files (size 25, taper 0.08) and Reciproc R25 files (size 25, taper 0.08) were operated in a reciprocating motion using preset adjustments, while One Shape files (size 25, taper 0.06) were used with in and out movements without pressure at a 400 tr/min rotation speed and 2. torque. In total, 5 ml of 2.5% NaOCl was used for irrigation of the canals. Before filling, each canal was flushed with saline solution and then dried with paper points. Prepared roots for each rotary system (n = 50/group) were divided randomly into the five groups (n = 10/group) based on the filling technique used. A total 15 experimental groups were assessed, and the teeth were then filled, as follows. For all filling techniques, Endoplus (President Dental, Duisburg, Germany) sealer was used.

Matched-single-cone filling

The root canals were filled with a size 25# specific single GP cone according to the NiTi file. (size 25# 0.08 tapered single GP cone (VDW, Munich, Germany) for Reciproc instrumentation group, size 25# 0.08 tapered single GP cone (Dentsply Maillefer, Switzerland, Brazil) for WaveOne instrumentation group, and size 25# 0.06 tapered single GP cone (MicroMega, Besancon, France) for One Shape instrumentation group).

Cold lateral compaction with a matched gutta-percha cone

A size 25# specific single GP cone with sealer-coated 0.02/20 accessory cones were used for filling of root canals.

Thermafil filling

For Reciproc; size R25 Guttafusion obturators, for WaveOne; 25 thermafil plastic obturators, for One Shape; 25 Herofill obturators were used to obturate the root canals.System B/Obtura II warm vertical compactionThe apical third of root canal was filled with System B/Obtura II was used for the backfill filling of root canal.

Cold lateral compaction

Root canals were filled with cold lateral compaction with size 25# standardized GP cones and accessory GP cones.

All fillings were completed by the same endodontist (A.D.), who was proficient in all filling techniques used. The specimens were stored in an incubator for 2 weeks at 37°C and 100% humidity to allow the sealer to set completely.

Root sectioning and image capture and analysis

A low-speed diamond-coated saw was used to section the roots horizontally at coronal, middle, and apical level with continuous water irrigation to prevent overheating (EXAKT 300 CL, Exakt Apparatbau, Norderstad, Germany). Sections were viewed at ×40 magnification using a stereomicroscope (Olympus SZ61, Olympus Corp., Tokyo, Japan) and digital images were captured with a digital camera (QImaging, BC, Canada) that attached to the stereomicroscope. On these digital images of each segment, the total area of each canal section and the areas of its contents (GP, sealer, voids) were measured in a metric system using the Image J (National Institutes of Health, Bethesda, MD, USA). The areas of GP, sealer, and voids were converted to percentages of the total area. The analysis of these cross-sections was performed by a second examiner who was blinded with respect to the experimental groups. For each section, measurements were repeated 3 times and the means were calculated. The accuracy rate of the examiners was 0.84 according to a correlation test.

Statistical analysis

For each continuous variable, normality was checked. Because the data were not distributed normally, an appropriate nonparametric test was used. Comparisons between groups were applied using Student’s t-test or one-way ANOVA for normally distributed data. The Mann-Whitney U-test or Kruskal-Wallis test was used when variables were not normally distributed. A univariate general linear model was applied to evaluate the related factors. If there were significant differences among the groups, the analysis was continued with post hoc comparisons using Tamhane’s, Dunnett’s, or Mann-Whitney U-test. Bonferroni’s correction was applied (P < 0.05/n; where n = a number of comparisons) when multiple comparisons were performed. Results are presented as means ± standard deviation. All reported P values are two-tailed. The SPSS statistical software (version 20.0; SPSS Inc., Chicago, IL, USA) was used for statistical analysis of the data.

   Results Top

The distribution of the PGFAs, the percentage of sealer-filled areas (PSFAs), and voids levels regarding to the instrumentation techniques, filling techniques, and localization groups were shown in [Table 1]. There was no significant difference in voids levels among the instrumentation groups (P = 0.333), there were significant differences between the groups according to the PGFA and PSFA levels (P < 0.001 for all comparisons). Reciproc instrumentation group demonstrated the highest PGFA and lowest PSFA level compare to the Wave One and One Shape instrumentation group.
Table 1: The distribution of the PGFA, PSFA and voids levels regarding to the instrumentation techniques, obturation techniques and localization groups

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The distribution of the PGFA, PSFA, and voids levels regarding to the subgroups were shown in [Table 2]. Significant differences were found among the five obturation technique groups in the distribution of GP, sealer, and voids (P = 0.0001, P = 0.0001, and P = 0.001, respectively). Canals filled with the System B + Obtura technique showed the highest PGFA and lowest PSFA and canals those filled with the matched-single-cone technique showed the highest PSFA and lowest PGFA.
Table 2: The distribution of the PGFA, PSFA and voids levels regarding to the subgroups

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Regarding localization, the ratio of PGFA was lower at the apical localization than the middle or coronal localization (P = 0.050). There were no significant differences among the localization groups according to PSFA and voids levels (P = 0.964 and P = 0.101, respectively). The distribution of the PGFA, PSFA, and voids levels regarding to the subgroups were shown in [Table 2]. The System B + Obtura technique showed the highest PGFA and lowest PSFA and voids levels in all instrumentation techniques groups. Hence, univariate general linear model analyses revealed that there is no significant interaction between instrumentation techniques and localization groups. There is significant interaction between obturation and instrumentation techniques and localization groups. Representative cross-section images from each experimental group at the middle level are shown in [Figure 1].
Figure 1: Cross-section of the different root canal filling techniques. (1) Matched-single-cone; (2) lateral compaction with matched gutta-percha cone; (3) Thermafil; (4) System B/Obtura II; and (5) lateral compaction with standardized gutta-percha cones which are instrumented with (a) WaveOne, (b) One Shape, (c) Reciproc file systems

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   Discussion Top

In this study, the Reciproc instrumentation group demonstrated higher PGFA and lower PSFA compared to the WaveOne and One Shape instrumentation groups in this study. Reciproc, WaveOne, and One Shape systems attempt to provide a single-file shaping technique without considering the length, diameter, or curvature of any given canal. In the current study, single-file systems with a tip diameter equivalent to a size of 25 file were selected for all instrumentation groups, even though Reciproc, WaveOne, and One Shape files have variable tapers (respectively, 0.08 taper, 0.08 taper, and 0.06 taper). The study outcome of the Reciproc group might be due to the cross-sectional design of the Reciproc file, which has a double cutting edge S-shaped geometry that provides high cutting efficacy.[11] Canals prepared with Reciproc file system might be filled and condensed better than those prepared with other file systems.

Moreover, when analyzing the localization, the ratio of PGFA was lower at the apical level than at the middle or coronal level (P < 0.05) for all groups investigated. This is possibly due to the oval shape or complex anatomy of distal canals of mandibular molars stemming from irregular canal shape and inadequate biomechanical preparation, which might be negatively affecting the filling quality. The percentage of voids for all groups was very low in this study, but the Thermafil technique had statistically more voids than the System B + Obtura obturation technique due to the root canal anatomy. Filling with one carrier was not sufficient for oval-shaped canals, so irregular canals should be filled with two carriers to decrease the sealer and void percentage.

System B + Obtura filling technique demonstrated the highest PGFA and lowest PSFA since heating and plugging of the GP significantly increased the sealing of lateral and accessory canals.[12] Thus, the hypothesis of this study was accepted. However, this technique showed decreased PGFA values and increased PSFA values at the apical level. This may be arisen because the GP in the apical portion of the root canal had not been softened adequately by heat and thereby GP adaptation was not performed. In parallel with this result, Cathro and Love[5] reported that the System B/Obtura II technique resulted in PGFA values of 99.5%, 99.4%, 100%, and 100% at 2, 4, 6, and 8 mm from apex, respectively, in standard resin canals.

Matched-single-cone filling is another filling technique which has been developed to minimize sealer content through the use of GP cones that closely match the geometry of the NiTi instrumentation systems.[13] However, there is doubt regarding the manufacturers’ claims for the supposed close matching of instruments and cone shapes because the files and cones often do not seem to match.[14] Some studies have evaluated the quality of these single-cone fillings in terms of sealing ability,[15],[16] bond strength,[17] radiographic quality,[9] PGFA, and PSFA.[7] Schäfer et al.,[7] reported similar PGFAs and PSFAs in canals filled either with lateral compaction of matched GP cones, warm vertical compaction, or lateral compaction of standardized GP cones at all levels evaluated, although the single-cone obturation resulted in a significantly lower values for GP and higher for sealer-filled areas. Another study by Pommel and Camps,[18] showed that the matched-single-cone technique produced the greatest amount of apical leakage. These studies are inconsistent with the current study but contradictory to this study Gordon et al.,[19] showed no significantly difference between the single-cone and the lateral condensation. In addition, Tasdemir et al.,[20] suggested that the matched-single-cone technique with tapered GP cones may yield better filling than the lateral compaction technique, at a level 2 mm from the apex. Another in vivo study reported no significant difference in treatment outcome between the matched-single-cone and lateral compaction techniques after 6–18 months.[21]

These obviously inconsistent results may be due to the shape of original canal, the differences in specimen preparation, the experimental protocols, or storage conditions. The use of single-cone obturation should be limited to round canals, because in irregular-shaped canals the use of single cones may result in voids[22] or greater volumes of sealer.[9] Therefore, small diameter and minimally curved mesial and distal roots of upper molars and the mesial roots of lower molars would be appropriate for this technique. In this study, the techniques were practiced extensively beforehand, and all specimens were prepared and filled by one operator to reduce variability. In order to reduce the possibility of deformation or fracture, each instrument was used to prepare only three root canals, and the sectioning was performed under water-cooling. When cutting the teeth, smearing of the filling on the section surface might occur despite the fact that water-cooling was used. Unnoticed smearing might have influenced accurate measurement of small void areas. In the present study, distal root with one single root canal were selected, however, this may have variation is in terms of outline, running form circular to oval being more or less extended in a faciolingual aspect. This is the limitation of this study. However, the teeth were randomly selected for experimental groups.

The optimum obturation technique must be used to ensure the integrity of the root canal filling in the few apical millimeters, which is regarded as the most crucial factor in the success of endodontic therapy. In order to ensure the long-term success of endodontic treatment, the root canal system should be obturated effectively, both coronally and apically.

   Conclusions Top

The distribution of the filling material was influenced by the preparation technique. Moreover, vertical compaction of warm GP achieves a better-quality filling in oval canals, than either matched-single-cone, the cold lateral compaction, or Thermafil techniques. Clinicians should be aware of the structure of the root canal before or during root canal treatment.


We would like to thank to Professor, Gulsah Seydaoglu for statistical analyses.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

   References Top

Bergmans L, Van Cleynenbreugel J, Wevers M, Lambrechts P. echanical root canal preparation with NiTi rotary instruments: Rationale, performance and safety. Status report for the American Journal of Dentistry. Am J Dent 2001;14:324-33.  Back to cited text no. 1
Alhashimi RA, Foxton R, Romeed S, Deb S. An in vitro assessment of gutta-percha coating of new carrier-based root canal fillings. Scientific World Journal 2014;2014:239754.  Back to cited text no. 2
Schäfer E, Köster M, Bürklein S. Percentage of gutta-percha-filled areas in canals instrumented with nickel-titanium systems and obturated with matching single cones. J Endod 2013;39:924-8.  Back to cited text no. 3
Hale R, Gatti R, Glickman GN, Opperman LA. Comparative analysis of carrier-based obturation and lateral compaction: A retrospective clinical outcomes study. Int J Dent 2012;2012:954675.  Back to cited text no. 4
Cathro PR, Love RM. Comparison of microseal and system B/obtura II obturation techniques. Int Endod J 2003;36:876-82.  Back to cited text no. 5
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Wu MK, Bud MG, Wesselink PR. The quality of single cone and laterally compacted gutta-percha fillings in small and curved root canals as evidenced by bidirectional radiographs and fluid transport measurements. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:946-51.  Back to cited text no. 8
Kontakiotis EG, Wu MK, Wesselink PR. Effect of sealer thickness on long-term sealing ability: A 2-year follow-up study. Int Endod J 1997;30:307-12.  Back to cited text no. 9
Tronstad L, Asbjørnsen K, Døving L, Pedersen I, Eriksen HM. Influence of coronal restorations on the periapical health of endodontically treated teeth. Endod Dent Traumatol 2000;16:218-21.  Back to cited text no. 10
Capar ID, Ertas H, Ok E, Arslan H. Comparison of single cone obturation performance of different novel nickel-titanium rotary systems. Acta Odontol Scand 2014;72:537-42.  Back to cited text no. 11
Robberecht L, Colard T, Claisse-Crinquette A. Qualitative evaluation of two endodontic obturation techniques: Tapered single-cone method versus warm vertical condensation and injection system: An in vitro study. J Oral Sci 2012;54:99-104.  Back to cited text no. 12
Zmener O, Pameijer CH, Macri E. Evaluation of the apical seal in root canals prepared with a new rotary system and obturated with a methacrylate based endodontic sealer: An in vitro study. J Endod 2005;31:392-5.  Back to cited text no. 13
Chesler MB, Tordik PA, Imamura GM, Goodell GG. Intramanufacturer diameter and taper variability of rotary instruments and their corresponding gutta-percha cones. J Endod 2013;39:538-41.  Back to cited text no. 14
Yilmaz Z, Deniz D, Ozcelik B, Sahin C, Cimilli H, Cehreli ZC, et al. Sealing efficiency of BeeFill 2in1 and system B/obtura II versus single-cone and cold lateral compaction techniques. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e51-5.  Back to cited text no. 15
Tasdemir T, Er K, Yildirim T, Buruk K, Celik D, Cora S, et al. Comparison of the sealing ability of three filling techniques in canals shaped with two different rotary systems: A bacterial leakage study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e129-34.  Back to cited text no. 16
Pawinska M, Kierklo A, Tokajuk G, Sidun J. New endodontic obturation systems and their interfacial bond strength with intraradicular dentine – Ex vivo studies. Adv Med Sci 2011;56:327-33.  Back to cited text no. 17
Pommel L, Camps J. In vitro apical leakage of system B compared with other filling techniques. J Endod 2001;27:449-51.  Back to cited text no. 18
Gordon MP, Love RM, Chandler NP. An evaluation of 0.06 tapered gutta-percha cones for filling of 0.06 taper prepared curved root canals. Int Endod J 2005;38:87-96.  Back to cited text no. 19
Tasdemir T, Yesilyurt C, Ceyhanli KT, Celik D, Er K. Evaluation of apical filling after root canal filling by 2 different techniques. J Can Dent Assoc 2009;75:201a-201d.  Back to cited text no. 20
Friedman S, Löst C, Zarrabian M, Trope M. Evaluation of success and failure after endodontic therapy using a glass ionomer cement sealer. J Endod 1995;21:384-90.  Back to cited text no. 21
Bergmans L, Moisiadis P, De Munck J, Van Meerbeek B, Lambrechts P. Effect of polymerization shrinkage on the sealing capacity of resin fillers for endodontic use. J Adhes Dent 2005;7:321-9.  Back to cited text no. 22


  [Figure 1]

  [Table 1], [Table 2]


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